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Please use this identifier to cite or link to this item: http://hdl.handle.net/10625/50250

Title: Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya
Authors: Kang’ethe, Erastus K.
Mulinge, Erastus K.
Skilton, Robert A.
Njahira, Moses
Monda, Joseph G.
Keywords: CRYPTOSPORIDIUM
GENOTYPING
DAIRY FARMING
KENYA
URBAN DAIRY PRODUCTION
ZOONOTIC DISEASE
FOOD-BORNE DISEASE
LIVESTOCK
Issue Date: 15-Jul-2012
Publisher: Springer
Citation: Kang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., et al. (2012). Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production, SI.doi:10.1007/s11250-012-0202-5
Abstract: A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twentyfive samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.
Description: This paper is part of a special supplement on assessing and managing urban zoonoses and food-borne disease in two African cities (Nairobi, Kenya and Ibadan, Nigeria).
URI: http://hdl.handle.net/10625/50250
ISSN: 0049-4747
Project Number: 103075
Project Title: Health Risk Analysis of Cryptosporidiosis and other Hazards in Urban Smallholder Dairy Production (Kenya)
Document Delivery: This document is not available in the IDRC Digital Library / Ce document n'est pas disponible dans la Bibliothèque numérique du CRDI
Appears in Collections:IDRC Research Results / Résultats de recherches du CRDI
2010-2019 / Années 2010-2019
Research Results (Ecohealth) / Résultats de recherches (Écosanté)
Sub-Saharan Africa / Afrique subsaharienne
Agricultural Transformation / Transformation agricole
Urban Ecosystems / Ecosystèmes urbains
Transdisciplinarity / Transdisciplinarité

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