Attribution: Please use this identifier to share, cite, or link to this item: http://hdl.handle.net/10625/49951
Title: Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide
Authors: Altman, E
Fernandez, H
Chandan, V
Harrison, B A
Schuster, M W
Keywords: CYTOTOXIN-ASSOCIATED ANTIGEN
HORSERADISH PEROXIDASE
LEWIS
WHOLE-CELL INDIRECT ELISA
HELICOBACTER PYLORI
TRIFUCOSYL LEWIS B ANTIGEN
Date: 2008
Citation: Altman, E., Fernandez, H., Chandan, V., Harrison, B. A., Schuster, M. W., Rademacher, L. O., & Toledo, C. (2008). Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide. Journal of Medical Microbiology, 57 (5): 585-591. doi: 10.1099/jmm.0.47783-0
Abstract: Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Lex) and Ley epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Lea and Leb structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Lex and/or Ley epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Leb blood group determinants, in addition to type 2 Lex and Ley structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).
ISSN: 0022-2615
1473-5644
URI: http://hdl.handle.net/10625/49951
Project Number: 102172
Project Title: Operational Research: Canadian International Immunization Initiative - Phase II (CIII2)
Copyright: Society for General Microbiology (SGM)
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