IDL-BNC @ IDRC >
IDRC / CRDI >
IDRC Research Results / Résultats de recherches du CRDI >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10625/34506

Title: Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan
Authors: Chabane, K.
Abdalla, O.
Sayed, H.
Valkoun, J.
Keywords: MICROSATELLITES
EST-SSR
SPECIES DISCRIMINATION
LANDRACES
REMOTE SENSING
WHEAT
BIODIVERSITY
AFGHANISTAN
Issue Date: 2007
Publisher: Springer, Dordrecht, NL
Abstract: Abstract Accurate and reliable means for identification are necessary to assess the discrimination between landraces of tetraploid wheat [T. turgidum L. subsp. durum (Desf.) Husn.] and hexaploid wheat (T. aestivum L. em. Thell.). In Afghanistan, farmers usually cultivate mixed landraces, and thus distinction between bread and durum is difficult. A set of 18 microsatellites derived from the DuPont ESTdatabase were used to describe genetic diversity in a sample of 82 Afghan wheat landraces. A total of 101 alleles were detected, with allele number per locus ranging from 2 to 13, and a mean allele number of 6.31. The percentage of polymorphic loci was 89%. The EST-SSRs markers showed different level of gene diversity: the highest Polymorphism Information Content value (0.921) was observed with DuPw 221. Our results demonstrated that with a reasonable number of expressed sequences target microsatellites (EST-SSRs) it is possible to discriminate between T. durum and T. aestivum species of wheat germplasm. Our results showed that ESTdatabases could be a useful source for speciesspecific markers and have the potential for new genic microsatellites markers that could enhance screening germplasm in gene banks.
URI: http://hdl.handle.net/10625/34506
Project Number: 101344
Project Title: Strengthening Seed Systems for Food Security in Afghanistan
Document Delivery: To enquire about document delivery, contact the IDRC Library : library@idrc.ca or 613-696-2578 / Pour plus de renseignements sur la livraison de documents, communiquer avec la bibliothèque du CRDI : bibliothèque@crdi.ca ou 613-696-2578
Appears in Collections:2000-2009 / Années 2000-2009
Asia / Asie
IDRC Research Results / Résultats de recherches du CRDI
Research Results (RPE) / Résultats de recherches (PRE)

Files in This Item:

File Description SizeFormat
126833.pdf
156.23 kBAdobe PDF
IDRC staff can log in using their network password; non-IDRC staff can request this document.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback